OrangerV1, Main, Exploration, bibRecord, 001215

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Identifieur interne : 001215 ( Main/Exploration ); précédent : 001214; suivant : 001216

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Auteurs : Jean-Paul Couillerot [France] ; David Windels [Suisse] ; Franck Vazquez [Suisse] ; Jean-Claude Michalski [France] ; Jean-Louis Hilbert [France] ; Anne-Sophie Blervacq [France]

Source :

Plant Signaling & Behavior [ 1559-2316 ] ; 2012.

RBID : PMC:3357352

Abstract

Somatic embryogenesis (SE) in Cichorium involves dedifferentiation and redifferentiation of single cells and can be induced by specific in vitro culture conditions. We have tested the effect of various treatments on the incidence of SE (ISE) of an interspecific embryogenic hybrid (C. endivia x C. intybus) and of different commercial chicories (C. endivia and C. intybus) that are typically recalcitrant to SE in standard culture conditions. We found that the ISE of the hybrid is significantly increased by pretreatment of tissues by submersion in solutions of glycerol, abscisic acid, spermine, putrescine or of combinations of these compounds. Interestingly, the most efficient of these pretreatments also had an unexpectedly high effect on the ISE of the C. intybus cultivars. The ISE of the hybrid and of the commercial chicories were increased when explants were co-cultured with highly embryogenic chicory explants or when they were cultured in conditioned medium. These observations established that unidentified SE-promoting factors are released in the culture medium. HPLC analyses of secreted Arabino-Galactan Proteins (AGPs), which are known to stimulate SE, did not allow identifying a fraction containing differentially abundant AGP candidates. However, pointing to their role in promoting SE, we found that the hybrid had a drastically higher ISE when amino sugars and L-Proline, the putative precursors of secreted AGPs, were both added to the medium.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357352

DOI: 10.4161/psb.7.1.18637
PubMed: 22301978
PubMed Central: 3357352

Affiliations:

France, Suisse

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p>Somatic embryogenesis (SE) in <italic>Cichorium</italic>
 involves dedifferentiation and redifferentiation of single cells and can be induced by specific in vitro culture conditions. We have tested the effect of various treatments on the incidence of SE (ISE) of an interspecific embryogenic hybrid (<italic>C. endivia</italic>
 x <italic>C. intybus</italic>
) and of different commercial chicories (<italic>C. endivia</italic>
 and <italic>C. intybus</italic>
) that are typically recalcitrant to SE in standard culture conditions. We found that the ISE of the hybrid is significantly increased by pretreatment of tissues by submersion in solutions of glycerol, abscisic acid, spermine, putrescine or of combinations of these compounds. Interestingly, the most efficient of these pretreatments also had an unexpectedly high effect on the ISE of the <italic>C. intybus</italic>
 cultivars. The ISE of the hybrid and of the commercial chicories were increased when explants were co-cultured with highly embryogenic chicory explants or when they were cultured in conditioned medium. These observations established that unidentified SE-promoting factors are released in the culture medium. HPLC analyses of secreted Arabino-Galactan Proteins (AGPs), which are known to stimulate SE, did not allow identifying a fraction containing differentially abundant AGP candidates. However, pointing to their role in promoting SE, we found that the hybrid had a drastically higher ISE when amino sugars and L-Proline, the putative precursors of secreted AGPs, were both added to the medium.</p>
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Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

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Abstract

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